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Antimicrobial activity of multi-mode periosteum scaffolds with different NIR irradiation strategies. A. Representative images of MRSA and E. coli colonies with different treatments. Quantitative analysis of MRSA ( B ) and E. coli ( C ) colonies in (A), respectively (n = 3). Results are presented as means ± SD. Samples were subjected to one-way ANOVA with Tukey's post hoc test. Ns ( p > 0.05), ∗∗ p < 0.01. D. Representative SEM images of MRSA and E. coli colonies with different treatments. Scale bar: 1 μm. E. Live/dead staining of MRSA and E. coli colonies in different treatment groups. Scale bar: 100 μm. F. Confocal microscopy image of MRSA biofilm. Scale bar: 100 μm. Protein leakage statistics for MRSA ( G ) and E. coli ( H ), respectively (n = 3). Results are presented as means ± SD. Samples were subjected to one-way ANOVA with Tukey's post hoc test. ∗∗ Significant differences of group M + CI vs. NC, M, and M + II, p < 0.01.
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Antimicrobial activity of multi-mode periosteum scaffolds with different NIR irradiation strategies. A. Representative images of MRSA and E. coli colonies with different treatments. Quantitative analysis of MRSA ( B ) and E. coli ( C ) colonies in (A), respectively (n = 3). Results are presented as means ± SD. Samples were subjected to one-way ANOVA with Tukey's post hoc test. Ns ( p > 0.05), ∗∗ p < 0.01. D. Representative SEM images of MRSA and E. coli colonies with different treatments. Scale bar: 1 μm. E. Live/dead staining of MRSA and E. coli colonies in different treatment groups. Scale bar: 100 μm. F. Confocal microscopy image of MRSA biofilm. Scale bar: 100 μm. Protein leakage statistics for MRSA ( G ) and E. coli ( H ), respectively (n = 3). Results are presented as means ± SD. Samples were subjected to one-way ANOVA with Tukey's post hoc test. ∗∗ Significant differences of group M + CI vs. NC, M, and M + II, p < 0.01.
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Antimicrobial activity of multi-mode periosteum scaffolds with different NIR irradiation strategies. A. Representative images of MRSA and E. coli colonies with different treatments. Quantitative analysis of MRSA ( B ) and E. coli ( C ) colonies in (A), respectively (n = 3). Results are presented as means ± SD. Samples were subjected to one-way ANOVA with Tukey's post hoc test. Ns ( p > 0.05), ∗∗ p < 0.01. D. Representative SEM images of MRSA and E. coli colonies with different treatments. Scale bar: 1 μm. E. Live/dead staining of MRSA and E. coli colonies in different treatment groups. Scale bar: 100 μm. F. Confocal microscopy image of MRSA biofilm. Scale bar: 100 μm. Protein leakage statistics for MRSA ( G ) and E. coli ( H ), respectively (n = 3). Results are presented as means ± SD. Samples were subjected to one-way ANOVA with Tukey's post hoc test. ∗∗ Significant differences of group M + CI vs. NC, M, and M + II, p < 0.01.
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Antimicrobial activity of multi-mode periosteum scaffolds with different NIR irradiation strategies. A. Representative images of MRSA and E. coli colonies with different treatments. Quantitative analysis of MRSA ( B ) and E. coli ( C ) colonies in (A), respectively (n = 3). Results are presented as means ± SD. Samples were subjected to one-way ANOVA with Tukey's post hoc test. Ns ( p > 0.05), ∗∗ p < 0.01. D. Representative SEM images of MRSA and E. coli colonies with different treatments. Scale bar: 1 μm. E. Live/dead staining of MRSA and E. coli colonies in different treatment groups. Scale bar: 100 μm. F. Confocal microscopy image of MRSA biofilm. Scale bar: 100 μm. Protein leakage statistics for MRSA ( G ) and E. coli ( H ), respectively (n = 3). Results are presented as means ± SD. Samples were subjected to one-way ANOVA with Tukey's post hoc test. ∗∗ Significant differences of group M + CI vs. NC, M, and M + II, p < 0.01.
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Antimicrobial activity of multi-mode periosteum scaffolds with different NIR irradiation strategies. A. Representative images of MRSA and E. coli colonies with different treatments. Quantitative analysis of MRSA ( B ) and E. coli ( C ) colonies in (A), respectively (n = 3). Results are presented as means ± SD. Samples were subjected to one-way ANOVA with Tukey's post hoc test. Ns ( p > 0.05), ∗∗ p < 0.01. D. Representative SEM images of MRSA and E. coli colonies with different treatments. Scale bar: 1 μm. E. Live/dead staining of MRSA and E. coli colonies in different treatment groups. Scale bar: 100 μm. F. Confocal microscopy image of MRSA biofilm. Scale bar: 100 μm. Protein leakage statistics for MRSA ( G ) and E. coli ( H ), respectively (n = 3). Results are presented as means ± SD. Samples were subjected to one-way ANOVA with Tukey's post hoc test. ∗∗ Significant differences of group M + CI vs. NC, M, and M + II, p < 0.01.
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Antimicrobial activity of multi-mode periosteum scaffolds with different NIR irradiation strategies. A. Representative images of MRSA and E. coli colonies with different treatments. Quantitative analysis of MRSA ( B ) and E. coli ( C ) colonies in (A), respectively (n = 3). Results are presented as means ± SD. Samples were subjected to one-way ANOVA with Tukey's post hoc test. Ns ( p > 0.05), ∗∗ p < 0.01. D. Representative SEM images of MRSA and E. coli colonies with different treatments. Scale bar: 1 μm. E. Live/dead staining of MRSA and E. coli colonies in different treatment groups. Scale bar: 100 μm. F. Confocal microscopy image of MRSA biofilm. Scale bar: 100 μm. Protein leakage statistics for MRSA ( G ) and E. coli ( H ), respectively (n = 3). Results are presented as means ± SD. Samples were subjected to one-way ANOVA with Tukey's post hoc test. ∗∗ Significant differences of group M + CI vs. NC, M, and M + II, p < 0.01.

Journal: Bioactive Materials

Article Title: A near-infrared regulated programmable multi-mode periosteum scaffold for sequential healing of infected bone defects

doi: 10.1016/j.bioactmat.2026.03.046

Figure Lengend Snippet: Antimicrobial activity of multi-mode periosteum scaffolds with different NIR irradiation strategies. A. Representative images of MRSA and E. coli colonies with different treatments. Quantitative analysis of MRSA ( B ) and E. coli ( C ) colonies in (A), respectively (n = 3). Results are presented as means ± SD. Samples were subjected to one-way ANOVA with Tukey's post hoc test. Ns ( p > 0.05), ∗∗ p < 0.01. D. Representative SEM images of MRSA and E. coli colonies with different treatments. Scale bar: 1 μm. E. Live/dead staining of MRSA and E. coli colonies in different treatment groups. Scale bar: 100 μm. F. Confocal microscopy image of MRSA biofilm. Scale bar: 100 μm. Protein leakage statistics for MRSA ( G ) and E. coli ( H ), respectively (n = 3). Results are presented as means ± SD. Samples were subjected to one-way ANOVA with Tukey's post hoc test. ∗∗ Significant differences of group M + CI vs. NC, M, and M + II, p < 0.01.

Article Snippet: Methicillin-resistant Staphylococcus aureus (MRSA, ATCC 43300) and Escherichia coli (ATCC 25922) were purchased from the American Type Culture Collection (ATCC).

Techniques: Activity Assay, Irradiation, Staining, Confocal Microscopy